Our Process

Begin by creating a formal submission on Agilent CrossLab, where you will also provide your payment information.

The Core will provide a custom quote for your project based on the service requested. The quote will vary depending on the sequencing needs of your particular samples.

Our typical microbiome sequencing costs are as follows:

Once you confirm the quote and provide payment, the Core will give you a custom report form with sample information.

We will also ship specialized tubes and custom labels directly to you. If you wish to send extracted DNA, you may ship it on ice in a sealed 96-well PCR plate.

At your location, you will be responsible for depositing 70mg of your sample into the specialized tubes and labeling them with the correct custom label.

In the case of shipped DNA, you will be responsible for shipping 20μl of DNA on a 96-well plate. The plate locations will be specified in the sample report.

Please note: The Core will NOT be responsible for samples placed into incorrect tubes/wells or sample information placed into incorrect spots on the sequence submission form.

The Core will confirm the arrival of all samples. Once all samples are received and confirmed, DNA extraction will begin.

The Core will process your samples utilizing the Promega Maxwell RSC 48 Instrument. We can process up to forty-eight (48) samples per hour.

After the Maxwell RSC 48 has finished extracting DNA from all samples, DNA samples will be quantified using the ThermoFisher Quant-It dsDNA High-Sensitivity Kit on a microplate reader.

Our team will process your extracted DNA based on your request: 16S or Metagenomic Sequencing.

We utilize state-of-the-art platforms for sequencing:

• 16S Sequencing: Performed on the Illumina HiSeq with PE250.

• Metagenomic Sequencing: Conducted on either the HiSeq 4000, NextSeq 500, or NovaSeq 6000 with PE150.

The platform used will be based on your specific sequencing depth requirements.

After sequencing, your raw and processed data will be uploaded to PubShare for you to download at your earliest convenience. The data will be de-multiplexed and ready for analysis.

Once sequencing is complete, you will be billed using the account number or purchase order (P.O.) number you provided during submission.