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16S SEQUENCING

  • DNA Is Extracted Utilizing Promega Max Prep & Promega Maxwell RSC 48 Instrument

  • The Core Will Use The 16S Illumina Amplicon Protocol From Earth Microbiome Project

  • Up To 960 Barcoded Primers Are Available

  • 515F-926R Primers Will Target V4 & V5 Regions

  • Library Pool Will Be Sequenced PE250 On The Illumina MiSeq, At 7pM & 15% PhiX

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