16S SEQUENCING
The Weill Cornell Medicine Microbiome Core offers advanced 16S rRNA Sequencing services for researchers seeking to profile microbial communities precisely. We are able to process a high-throughput number of samples using advanced technology and refined 16S rRNA Sequencing protocols. Our commitment to quality ensures robust and reproducible results tailored to your research needs.
Advanced DNA Extraction Methodology
To begin 16S rRNA Sequencing, we utilize our Promega Max Prep & Promega Maxwell RSC 48 robotics for optimal DNA extraction. This method ensures high-yield genomic DNA extraction from various samples, including environmental, human, mouse, or clinical sources.
Illumina Amplicon Protocol
Our 16S Sequencing pipeline employs the 16S Illumina Amplicon Protocol, established by the Earth Microbiome Project. This approach ensures the most efficient amplification of the 16S rRNA gene for subsequent sequencing, using up to 960 barcoded primers tailored for large-scale multiplexing.
The targeted primer set of 515F-926R focuses on the V4 and V5 regions of the 16S rRNA gene, enabling comprehensive detection of a wide range of bacterial and archaeal taxa.
High-Throughput Sequencing Capability
Library pools generated through our protocols are sequenced using the Illumina MiSeq platform, utilizing a 250bp paired-end (PE250) configuration at a concentration of 3 pM with 15% PhiX spike-in.
This high-throughput capability allows us to process hundreds of samples in parallel, ensuring efficient turnaround times while maintaining exceptional data quality.
